Sri Lankan Scientists Show Impact of Papaya Leaf on Blood


News from the island that looks like a papaya;
Papaya leaf extract protects blood from hemolysis, and Sri Lankan scientists Ranasinghe P, Ranasinghe P, Abeysekera WP, Premakumara GA, Perera YS, Gurugama P, Gunatilake SB believe that papaya leaf extract can fight …”disease process causing destabilization of biological membranes.” This category should include invasive cancers.

See referenced University of Colombo study:

Papaya Leaf Tea is Four Times More Protective of Cells than Vitamin C


New tests in the lab at Niger Delta University show that papaya leaf “tea” protects your red blood cells (erythrocytes) from free-radical damage (lipid peroxidation) better than ascorbic acid (manufactured vitamin C).

Reduction of hydrogen peroxide-induced erythrocyte damage by Carica papaya leaf extract.

Okoko T, et al. Show all Journal
Asian Pac J Trop Biomed. 2012 Jun;2(6):449-53. doi: 10.1016/S2221-1691(12)60074-4.

Biochemistry Programme, Department of Chemical Sciences, Niger Delta University, PMB 71, Wilberforce Island, Bayelsa State, Nigeria.

OBJECTIVE: To investigate the in vitro antioxidant potential of Carica papaya (C. papaya) leaf extract and its effect on hydrogen peroxide-induced erythrocyte damage assessed by haemolysis and lipid peroxidation.

METHODS: Hydroxyl radical scavenging activities, hydrogen ion scavenging activity, metal chelating activity, and the ferrous ion reducing ability were assessed as antioxidant indices. In the other experiment, human erythrocytes were treated with hydrogen peroxide to induce erythrocyte damage. The extract (at various concentrations) was subsequently incubated with the erythrocytes and later analysed for haemolysis and lipid peroxidation as indices for erythrocyte damage.

RESULTS: Preliminary investigation of the extract showed that the leaf possessed significant antioxidant and free radical scavenging abilities using in vitro models in a concentration dependent manner (P<0.05). The extract also reduced hydrogen peroxide induced erythrocyte haemolysis and lipid peroxidation significantly when compared with ascorbic acid (P<0.05). The IC50 values were 7.33 mg/mL and 1.58 mg/mL for inhibition of haemolysis and lipid peroxidation, respectively. In all cases, ascorbic acid (the reference antioxidant) possessed higher activity than the extract.

CONCLUSIONS: The findings show that C. papaya leaves possess significant bioactive potential which is attributed to the phytochemicals which act in synergy. Thus, the leaves can be exploited for pharmaceutical and nutritional purposes.


Cytokines Talk Tea


Dr Dang’s research on the effect of papaya leaf “tea” on cancer reviewed the now proven impact of T-1 type cytokines on communicating an immune response.

The article below explains how cytokines “talk tea”.

The immune-body cytokine network defines a social architecture of cell interactions
Ziv Frankenstein1,2, Uri Alon2 and Irun R Cohen*1

Background: Three networks of intercellular communication can be associated with cytokine secretion; one limited to cells of the immune system (immune cells), one limited to parenchymal cells of organs and tissues (body cells), and one involving interactions between immune and body cells (immune-body interface). These cytokine connections determine the inflammatory response to injury and subsequent healing as well as the biologic consequences of the adaptive immune response to antigens. We informatically probed the cytokine database to uncover the underlying network architecture of the three networks.
Results: We now report that the three cytokine networks are among the densest of complex networks yet studied, and each features a characteristic profile of specific three-cell motifs. Some legitimate cytokine connections are shunned (anti-motifs). Certain immune cells can be paired by their input-output positions in a cytokine architecture tree of five tiers: macrophages (MΦ) and B cells (BC) comprise the first tier; the second tier is formed by T helper 1 (Th1) and T helper 2 (Th2) cells; the third tier includes dendritic cells (DC), mast cells (MAST), Natural Killer T cells (NK-T) and others; the fourth tier is formed by neutrophils (NEUT) and Natural Killer cells (NK); and the Cytotoxic T cell (CTL) stand alone as a fifth tier. The three-cell cytokine motif architecture of immune system cells places the immune system in a super-family that includes social networks and the World Wide Web. Body cells are less clearly stratified, although cells involved in wound healing and angiogenesis are most highly interconnected with immune cells.
Conclusion: Cytokine network architecture creates an innate cell-communication platform that organizes the biologic outcome of antigen recognition and inflammation. Informatics sheds new light on immune-body systems organization.”

Papaya Leaf Tea Stimulates Macrophages


Scientists have discovered that papaya leaf tea stimulates macrophages, the “big eaters” in your body.

Macrophages clean up waste in your body including poisons, pathogens, and, of course, cancer cells.

In photo above the macrophage is extending its arms to engulf two pathogens.

A very good description of macrophages was published in Science Daily (below):

American Physiological Society (2010, August 27).

Macrophages: The ‘defense’ cells that help throughout the body. –ScienceDaily. Retrieved

Aug. 27, 2010 — The term “macrophage” conjures images of a hungry white blood cell gobbling invading bacteria. However, macrophages do much more than that: Not only do they act as antimicrobial warriors, they also play critical roles in immune regulation and wound-healing. They can respond to a variety of cellular signals and change their physiology in response to local cues.

“There has been a huge outpouring of research about host defense that has overshadowed the many diverse activities that these cells do all the time,” said Dr. Mosser. “We’d like to dispel the narrow notion that most people have that macrophages’ only role is defense, and expand it to include their role in homeostasis.”

Monocyte Differentiation
Macrophages exist in nearly all tissues and are produced when white blood cells called monocytes leave the blood and differentiate in a tissue-specific manner. The type of macrophage that results from monocyte differentiation depends on the type(s) of cytokines that these cells encounter. Cytokines are proteins produced by immune cells that can influence cell behavior and affect interactions between cells. For example, macrophages that battle microbial invaders arise in response to interferon-γ, a cytokine that is produced during a cellular immune response involving helper T-cells and the factors they produce. These macrophages are considered to be “classically activated.”
However, when monocytes differentiate in response to stimuli such as prostaglandins or glucocorticoids, the resulting macrophages will assume a “regulatory” phenotype. Alternately, wound-healing macrophages arise when monocytes differentiate in response to interleukin-4, a cytokine which is released during tissue injury…

Immune Regulation
Immune-regulating macrophages produce high levels of the cytokine interleukin-10, which helps suppress the body’s immune response. Suppressing an immune response may seem counter-intuitive, but in the later stages of immunity it comes in handy because it limits inflammation.

According to Dr. Mosser, immune-regulating macrophages may hold the key to developing treatments for autoimmune diseases such as multiple sclerosis or rheumatoid arthritis. The focus of new research is on reprogramming the macrophages to assume a regulatory phenotype and prevent autoimmunity, he said.
There is broad potential for exploiting different stages of macrophage activation, Dr. Mosser added. “It might be possible to manipulate macrophages to make better vaccines, prevent immunosuppression, or develop novel therapeutics that promote anti-inflammatory immune responses.”

Harvard Graduate Has Patent Pending on Papaya Leaves for Cancer


Dr Nam Dang is a medical oncologist with expertise in the study and treatment of lymphoma and chronic lymphocytic leukemia (CLL). He is Professor and Deputy Chief of the Division of Hematology & Oncology and also is Director of the University of Florida Shands Cancer Center Clinical Trials Office. Dr. Dang received his Phd in Immunology from Harvard.

WE are excited about Dr Dang’s work on papaya leaves and greatly appreciate what he has shown in research on their effect against cancer(s). The Julia Ruffin Project focuses on promoting, growing, and researching papaya leaves. We offer papaya leaves grown from non-genetically modified seeds… just the natural product to markets. The Julia Ruffin Project is non-profit and is not creating additional products from the leaves (such as selling extracted leaf juice) so the patent-pending is not applicable to the young papaya leaves that we grow for market. Go to

Clinical Study Shows Effect on Breast Cancer

A clinical study in 2001 was performed on 2,339 breast cancer patients. The study was meant to determine the effect of oral enzymes (OE). Papaya Leaf Tea is a form of (OE) Oral Enzyme Therapy, with the most powerful food enzyme available.

A clear reduction in the side effects of radiotherapy and chemotherapy was documented in 74% of the test group and 55% of the control group. Analysis of survival, recurrence, and metastasis demonstrated a reduced number of events in the test group. There was evidence of a beneficial influence of OE on time to event, although the median observation time was too short in these breast cancer patients to draw definite conclusions. The safety component was judged in 98% of the test group and 76% of the control group as “very good” or “good”. In the total sample of 2,339 patients, the rate of OE-associated adverse reactions was 3.2%. All side effects were mild to moderate gastrointestinal symptoms.

Conclusion: Complementary treatment of breast cancer patients with OE improves the quality of life by reducing signs and symptoms of the disease and the side effects of adjuvant antineoplastic therapies. This epidemiological retrolective cohort analysis provides evidence that the patients may also gain benefit by a prolongation of the time to event for cancer recurrence, metastasis and survival. OE was generally well tolerated.

PMID: 11561873 [PubMed – indexed for MEDLINE]
-Beuth J, Ost B, Pakdaman A, Rethfeldt E, Bock PR, Hanisch J, Schneider B, Institute for Scientific Evaluation of Naturopathy, University of Cologne, Köln,
oSearch=11561873&ordinalpos=18&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Res ultsPanel.Pubmed_RVDocSum

We hope the supporters of Susan G Komen can read this study.



Thank you Dr. Nixon


Six years ago I felt a need to get the support of an oncologist in my research about clinical trials related to papaya leaf tea, papain, and related ethnomedicine. I looked up credentials of oncologists in various cities around where I lived in Savannah, GA. I decided to call Dr. Daniel Nixon who was at the time working in Charleston, SC.

I said the first time that I heard his “Hello”, “Hi, I am Virginia Robertson. I want to talk with you about papain and papaya leaf tea and its effect on cancer. I want you to read my research.” Dr Nixon said, “Who are you?” I said, “Virginia Robertson”. I didn’t have credentials. But the kind doctor sent me his email and I forwarded my research. Two weeks later he called me back and said that he thought I may have something.

Dr Nixon arranged for us to do some research with an associate of his in a lab that studied melanoma at Clemson. I sent samples. This positive result was one of the markers for me that helped me continue my pursuit. I want to thank Dr. Nixon for his help early on.

Currently Dr. Nixon works for Cancer Treatment Centers of America in the Western Region Medical Center near Phoenix. Dr. Nixon is a member of the Senior Executive Service for the United States of America, Nutrition Oncology Research Cooperative Agreement, American Federation for Clinical Research, American Medical Association, American Society of Clinical Oncology and American Association for Cancer Research.

“Thank you” from the Julia Ruffin Project