Lysis Corrected with Papaya Leaf in Lab


This is Lysis: (see image above)
Lysis refers to the breaking down of a cell, often by viral, enzymic, or osmotic mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a “lysate”.

Studies have shown that papaya leaf can help the body produce red blood cells and in this way cure a patient from Dengue Fever.

A curious group of scientists did an experiment with papaya leaf extract and red blood cells that showed lysis (hemolysis). In an amazing feat, the scientists showed that the papaya leaf actually restored the cells to health!

This research shows that the papaya leaf not only helps the body make red blood cells but can also heal the lysis! Does this show that the leaf may have an anti-Viral effect? This discovery may explain why fermented papaya (inc. papaya leaf) has shown effect against severe lysis in the form of cell defects such as Parkinson’s disease and Cystic Fibrosis, and even Cancer.

See study below:

In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

AUTHOR(S)Ranasinghe, Priyanga; Ranasinghe, Pathmasiri; Abeysekera, W. P. KaushalyaM.; Premakumara, G. A. Sirimal; Perera, Yashasvi S.; Gurugama, Padmalal; Gunatilake, Saman B.

October 29, 2012
Pharmacognosy Research;
Oct-Dec2012, Vol. 4 Issue 4, p196

Background: Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay.

Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy volunteers and patients with serologically confirmed current dengue infection were freshly collected and used in the assays. Fresh papaya leaves at three different maturity stages (immature, partly matured, and matured) were cleaned with distilled water, crushed, and the juice was extracted with 10 ml of cold distilled water. Freshly prepared cold water extracts of papaya leaves (1 ml containing 30 �l of papaya leaf extracts, 20 �l from 40% erythrocytes suspension, and 950 �l of phosphate buffered saline) were used in the heat-induced and hypotonicinduced hemolytic assays. In dose response experiments, six different concentrations (9.375, 18.75, 37.5, 75, 150, and 300 �g/ml) of freeze dried extracts of the partly matured leaves were used. Membrane stabilization properties were investigated with heat-induced and hypotonicityinduced hemolysis assays.

Results: Extracts of papaya leaves of all three maturity levels showed a significant reduction in heat-induced hemolysis compared to controls (P 0.05) different from one another. Heat-induced hemolysis inhibition activity did not demonstrate a linear dose response relationship. At 37.5 �g/ml concentration of the extract, a marked inhibition of hypotonicityinduced hemolysis was observed.

Conclusion: C. papaya L. leaf extracts showed a significant inhibition of hemolysis in vitro and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes.