Protease Enzyme Therapy for Cancer and Papaya Leaf

Scientists continue to work to define the reason why proteases like the ones in papaya leaf (papain and chymopapain) have shown to slow the growth of tumors.

“…we have discovered that proenzyme therapy, mandated first by John Beard nearly one hundred years ago, shows remarkable selective effects that result in growth inhibition of tumor cells with metastatic potential.”

-Proenzyme therapy of cancer. Novak JF, et al. Anticancer Res. 2005 Mar-Apr.

John Beard, a Scottish doctor, proved the effect of trypsin (a protease taken from a young cow) on halting the growth of tumors in humans by injecting part of the Pancreas of the young cow straight into human tumors.

Papain has been shown to be more powerful of a protease than trypsin.

What Type of Cancer Has Papaya Leaf Shown to Affect?

Sheikh Fauziya in his paper, “Papaya: Source for Anticancer”, created a summary of the types of cancer lines studied so far that papaya leaf has shown to affect:

Stomach Cancer
Pancreatic Cancer
Colon Cancer
Ovarian Cancer
Lymphoma Cancer
Breast Cancer
Neuroblastoma Cancer
Uterine Cancer
Leukemia Cancer
Cervical Cancer
Hepatocellular Cancer

-CIBTech Journal of Pharmaceutical Sciences ISSN: 2319–3891 (Online)
An Online International Journal Available at 2013 Vol.2 (1) January-March, pp.25-34/Sheikh and Krishnamurthy
Review Article
*Sheikh Fauziya and R Krishnamurthy
CG Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus Bardoli, Dist. Surat, Gujarat, India-394350
*Author for Correspondence…Krishnamurthy…Papaya…Anticancer…25-33.pdf

Papaya Leaves as Ethnomedicine

Below is a short-list of ten countries and their traditional medicinal uses of papaya leaves taken from a fantastic overview of current research on papaya leaves:
Carica Papaya Leaf Extracts – An Ethnomedicinal Boon
*Tatyasaheb Patil1, Snehal Patil2, Anuprita Patil3, Shreedevi Patil4
Published by
International Journal of Pharmacognosy and Phytochemical Research 2014; 6(2); 260-265

Table 2: Use of papaya leaves by various indigenous populations –Indigenous populations usually cure their sick using various plant products. So it would pave way for future research to understand the various uses of papaya leaves by different populations. *1-5

Places Researched:

East Indies
Ghana Ivory coast
French Guiana
Carribean region

Uses of carica papaya leaves:
Young leaves are cooked and eaten like spinach.
Crushed leaves may be used to tenderize meat. Also functions as a Vermifuge and a primitive soap substitute in laundering.
Dried leaves have been smoked to relieve asthma or as a tobacco substitute.
Purgative for stomach troubles
Purgative for horses
Treatment of genitor urinary ailments
Relief in nausea(during pregnancy)
Leaf are prepared in combination with Quassia amara, Euterpe oleracea and Citrus sp for the treatment of malarial fever
Heart tonic, febrifuge, vermifuge, colic fever, beri beri, abortion and asthma
Rheumatic complaints
Stomach troubles and cancer
To treat high blood pressure
Rheumatoid arthritis, constipation, jaundice, diabetes, dermatitis, hurt
Packages of dried, pulverized leaves are sold by “health food” stores for making tea (used as tumour destroying agent )
The fresh green tea acts as antiseptic and dried leaves are best as a tonic and blood purifier
Also used as digestive system promoter, chronic indigestion, weight loss, obesity, arteriosclerosis, high blood pressure and weakening of heart
Diuretic and Antihelminthic

1.Okoli RI, Aigbe O, Ohaju-Obodo JO, Mensah JK. Medicinal Herbs Used for Managing Some Common Ailments among Esan People of Edo State, Nigeria. Pakistan Journal of Nutrition 2007; 6 (5): 490-496.
2. Halberstein RA. Medicinal Plants: Historical and Cross-Cultural Usage Patterns. Ann Epidemiol 2005; 15: 686–699.
3. Ogbe FMD, Eruogun OL, Uwagboe M. Plants used for female reproductive health care in Oredo local government area. Nigeria Scientific Research and Essay 2009; 4: 120-130.
4. Rahmatullah M, Ferdausi D, Mollik MAH, Jahan R, Chowdhury MH, Haque WM. A survey of medicinal plants used by kavirajes of chalna area, khulna district, bangladesh. Afr. J. Trad. CAM 2010; 7: 91 – 97.
5. Teklehaymanot T, Giday M. Ethnobotanical study of medicinal plants used by people in Zegie Peninsula, Northwestern Ethiopia. Journal of Ethnobiology and Ethnomedicine 2007; 3: 12.,Vol6,Issue2,Article20.pdf

This blog is sponsored by the Julia Ruffin Project. The Project promotes, researches, and grows papaya leaf tea.
#alternativemedicine #cancer

Enzyme in Papaya Leaf, Papain, Inhibits Tumor Growth and Metastatis in Lab

Proteolytic Enzyme Therapy for cancerous tumors goes back to the success of Dr Beard in 1910 (Enzyme Therapy for Cancer). He retrieved the trypsin (a proteolytic enzyme) from young cows and injected it straight into the patient’s Tumor. This method showed success. Doctors who subsequently tried to mimic this procedure were discredited but they tried to retrieve trypsin from old cows, which by definition have less pancreatic enzymes due to their age. The theory of an “enzyme bank” that decreases as we age belongs to Dr Edward Howell, who proved this in a series of studies over years. (1945 +)

The enzyme in papaya leaves, papain, is a proteolytic enzyme that also seems to show a similar effect on tumors, as in this animal study below. Papain is a more potent proteolytic enzyme than our body’s trypsin.

Inhibition of tumor growth, invasion and metastasis in papain-immunized mice.

Bellelli A, Mattioni M, Rusconi V, Sezzi ML, Bellelli L.
Invasion Metastasis. 1990;10(3):142-69.

“The growth rate, invasion and metastasis of both the B16 melanoma and the Lewis lung carcinoma were inhibited in mice immunized with papain. These animals presented an increased mean survival time as compared to the tumor-bearing nonimmunized controls. Quantitative microscopy suggested that vasodilation and edema, associated with tumor invasion, are, at least partially, sustained by proteolytic enzymes, being strongly reduced when tumor cells were inoculated in papain-immunized mice.”

What We Call PAPAYA Leaves For Cancer!

The Julia Ruffin Project grows, researches, and promotes papaya leaf tea.

This is what we call “Papaya” around the world:

BURMESE: Thimbaw.
CZECH: Papaja.
ESTONIAN : Harilik papaia, Papaia.
FIJIAN: Oleti.
FRENCH: Papaye, Papayer.
GERMAN: Melonenbaum, Papayabaum.
GUJARATI: Papaiya, Papayi.
HAWAIIAN: He’i, Mikana, Milikana.
HINDI: Papeeta, Papiitaa.
ITALIAN: Papaia.
JAPANESE: Motukuwa, Papaia, Popoo.
KHMER: Ihong, Doeum lahong.
KOREAN: Pa pa ya.
MALAY: Betek, Betik, Gedang, Kates, Ketalah. Papaya.
MALAYALAM: Karamooza, Omakai, Omakaya.
MARATHI: Papai, Papaya, Popai.
PALAUAN: Babai, Bobai.
POLISH: Melonowiec właściwy, Papaja.
PORTUGUESE: Ababaia, Mamao, Papaia, Fruto de Mamoeiro, Papaeira.
PUNJABI: Katcha pepita.
RUSSIAN: Papaia.
SPANISH: Fruta bomba, Lechosa, Melon de arbol, Melon zapote, Papayero, Papayo, Papaya.
THAI: Loko, Malako, Malakor, Ma kuai thet, Sa kui se.
TONGAN: Lesi, Lesi fefine
URDU: Papiitaa, Pappeeta.

Papaya Leaf Increases Platelet Count and Red Blood Cell Count in Sri Lankan Study

Sri Lankan study published this year in the Asian Journal of Tropical Biomedicine reached this conclusion:

Fresh C. papaya leaf extract significantly increased the platelet and RBC counts in the test group as compared to controls. Therefore, it is very important to identify those chemicals of C. papaya leaves as it can be recommended to be used as a medication to boost thrombopoiesis and erythropoiesis in humans and in animals.

Does Carica papaya leaf-extract increase the platelet count? An experimental study in a murine model
Sinhalagoda Lekamlage Chandi Asoka

Dharmarathna1, Susiji Wickramasinghe2*, Roshitha Nilmini Waduge3, Rajapakse Peramune Veddikkarage Jayanthe Rajapakse1, Senanayake Abeysinghe Mudiyanselage Kularatne4
1Department of Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka 2Department of Parasitology, Faculty of Medicine, University of Peradeniya, Sri Lanka
3Department of Pathology, Faculty of Medicine, University of Peradeniya, Sri Lanka
4Department of Medicine, Faculty of Medicine, University of Peradeniya, Sri Lanka

Siti Zaleha Halim, Herbal Medicine Research Center, Institute for Medical Research, Jalan Pahang 50588, Kuala Lumpur, Malaysia.

This is a interesting study of the efferct of C. papaya leaves extract on the haematological and biochemical parameters in a murine model. The findings are impressive and useful for continuous study related to C. papaya. C. papaya leaf could be an alternative to treat dengue and malaria fever instead of prescription drugs.

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Current Research Shows Papaya Leaf Tea is Active Against Human Pathogens

Researchers just this year have published on the effect of papaya leaf tea (aqueous extract of papaya leaf) on human pathogens. Pakistani researcher Sherwani points out that we need to find a new bio active compound to combat infectious disease. In the study below his team showed effect of papaya leaf tea on seventeen human and plant pathogenic fungi. The study was published in the International Research Journal of Pharmacy 9/6/13.